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Q: Which is the first step involved in chymotrypsin mediated peptide bond hydrolysis?
Chymotrypsin reaction with its substrates takes place in 2 stages, an initial burst phase at the beginning of the reaction and steady state phase following MM kinetics. The mode of action is explained in 2 steps: * Acylation of substrate to form an acyl-enzyme intermediate. * Deacylation in order to return the enzyme to its original state. Hence the first step is acylation.